With Ln-staining, the sample preparation protocol becomes extremely simple:
JUST THREE STEPS...
staining itself (physiological conditions are desirable);
final rinse (to remove any of the remaining contrast)
...to make the sample ready for SEM observation in a low vacuum mode!
To undergo high vacuum SEM, your Ln-stained sample has to be carbon coated first. For that, let it dry at
room conditions for two hours after the staining procedure or place it under the vacuum of your microscope
for just a few seconds.