Breathing new life into your high-energySEM

Lanthanoid staining

for scanning electron microscopy of biological samples

 

Scanning (raster) electron microscopy (SEM) is used in a wide range of sciences and industries - from biology to materials science. However, it is in biology and medicine that SEM is now losing its utility due to low information value of its images (surface relief only, while the interior is particularly interesting) and complexity of sample preparation.

Our team has developed a simple method that solves these problems. First of all, it allows the study of internal structures of biological objects being free to choose the accelerating voltage and to use a back-scattered electron detector. It has also the benefit of faster sample preparation (1 hour instead of days) and more detailed images.

What do we offer?

We are happy to present you a new method of lanthanoid staining of biological samples for SEM that possesses the following advantages:

  • Not only the surface topography, but also the interior of the cells can be studied
  • Low-vacuum SEM can be done without prior fixation, or dehydration, or sputtering of the samples
  • Images obtained are, in many respects, similar to those of WETSEMTM, and yet, no expensive accessories are required
  • Samples are ready in less than an hour!
  • You no longer need toxic, volatile, or flammable chemicals in the lab

What good is it?

Well, at least you are now able to…

...take a look DEEPER than that of classic light microscopy,

Classic light microscopy of selectively stained samples is an easy option. However, if you aim to investigate the fine structure of cells and their interactions, you might find the resolution insufficient.

...take a view BROADER than that of super-resolution systems,

You can also turn to super-resolution fluorescent microscopy, but if you still want to get the idea of cell interactions in culture, you will definitely need more pixels within the field of view.

...and enjoy the WHOLE PICTURE without having to assemble a puzzle!

Moreover, with super-resolution microscopy, you always have to decide in advance which structures to visualize. And before you see the whole picture, you should put all the data together as if pieces of a jigsaw puzzle. Our method provides the opportunity to visualize nucleus membrane, nucleoli, cytoskeleton parts, vacuoles, intercellular contacts, filaments of locomotion structures, etc. - all at the same time!

Mitochondria in human astrocytes


Modified Image BioREE
Original Image Fluorescence
Carl ZEISS Axio Vert. A1
(x40)
Carl ZEISS EVO LS 10
BSE, 23 kV
Mito Tracker® Red contrast agent BioREE Contrast agent